Amphiphysin antibody (Internal Region)
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- Target See all Amphiphysin (AMPH) Antibodies
- Amphiphysin (AMPH)
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Amphiphysin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- Amphiphysin / AMPH
- Sequence
- KRKDESRISK AE
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1.
- Isotype
- IgG
- Top Product
- Discover our top product AMPH Primary Antibody
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anti-Amphiphysin (AMPH) (N-Term) antibody
AMPH Reactivity: Human WB, ICC, IHC (p), IF Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (C-Term) antibodyAMPH Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (AA 24-240) antibodyAMPH Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (AA 24-241) antibodyAMPH Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) antibodyAMPH Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (AA 2-15) antibodyAMPH Reactivity: Human, Mouse, Rat, Chicken, Hamster WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (Internal Region) antibodyAMPH Reactivity: Human, Mouse, Rat WB, ELISA, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-Amphiphysin (AMPH) (AA 336-370) antibodyAMPH Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB51675 unconjugated
anti-Amphiphysin (AMPH) (AA 140-168) antibodyAMPH Reactivity: Human WB Host: Rabbit Polyclonal RB41814 unconjugated
anti-Amphiphysin (AMPH) (AA 1-347) antibodyAMPH Reactivity: Human, Mouse, Rat WB, IHC, IP, ICC, IHC (p) Host: Mouse Monoclonal unconjugated
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.
Western Blot: Approx 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Amphiphysin (AMPH)
- Alternative Name
- AMPH (AMPH Products)
- Background
- AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen) , HGNC:471, AMPH1
- Gene ID
- 273, 218038
- NCBI Accession
- NP_001626, NP_647477
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