ADRB1 antibody (Internal Region)

Catalog No. ABIN185584

Product Details anti-ADRB1 Antibody

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADRB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: ADRB1

Sequence: ESDEARRCYN DPK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence ESDEARRCYNDPK, from the internal region of the protein sequence according to NP_000675.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 6 μg/mL.

Western Blot: This antibody is not recommended for use in Western blotting.

Peptide ELISA: antibody detection limit dilution 1:2000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plama membrane and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ADRB1 antibody (ABIN185584)

Wenzel, Schulze-Rothe, Müller, Wallukat, Haberland: "Difference between beta1-adrenoceptor autoantibodies of human and animal origin-Limitations detecting beta1-adrenoceptor autoantibodies using peptide based ELISA technology." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192615, (2018) (PubMed).

Wallukat, Prüss, Müller, Schimke: "Functional autoantibodies in patients with different forms of dementia." in: PLoS ONE, Vol. 13, Issue 3, pp. e0192778, (2018) (PubMed).

Wenzel, Schulze-Rothe, Haberland, Müller, Wallukat, Davideit: "Performance and in-house validation of a bioassay for the determination of beta1-autoantibodies found in patients with cardiomyopathy." in: Heliyon, Vol. 3, Issue 7, pp. e00362, (2017) (PubMed).

Wallukat, Haberland, Berg, Schulz, Freyse, Dahmen, Kage, Dandel, Vetter, Salzsieder, Kreutz, Schimke: "The first aptamer-apheresis column specifically for clearing blood of ?1-receptor autoantibodies." in: Circulation journal : official journal of the Japanese Circulation Society, Vol. 76, Issue 10, pp. 2449-55, (2012) (PubMed).

Haberland, Wallukat, Dahmen, Kage, Schimke: "Aptamer neutralization of beta1-adrenoceptor autoantibodies isolated from patients with cardiomyopathies." in: Circulation research, Vol. 109, Issue 9, pp. 986-92, (2011) (PubMed).

Wallukat, Muñoz Saravia, Haberland, Bartel, Araujo, Valda, Duchen, Diaz Ramirez, Borges, Schimke: "Distinct patterns of autoantibodies against G-protein-coupled receptors in Chagas' cardiomyopathy and megacolon. Their potential impact for early risk assessment in asymptomatic Chagas' patients." in: Journal of the American College of Cardiology, Vol. 55, Issue 5, pp. 463-8, (2010) (PubMed).

Target Details for ADRB1

Target: ADRB1 (Adrenergic, beta-1-, Receptor (ADRB1))

Alternative Name: ADRB1 (ADRB1 Products)

Synonyms: ADRB1R antibody, B1AR antibody, BETA1AR antibody, RHR antibody, RATB1AR antibody, X-Beta1AR antibody, BAR1 antibody, zgc:194728 antibody, zgc:194731 antibody, ADRB1 antibody, Adrb-1 antibody, beta-AR antibody, adrenoceptor beta 1 antibody, adrenoceptor beta 1 L homeolog antibody, adrenergic, beta-1-, receptor antibody, adrenergic receptor, beta 1 antibody, ADRB1 antibody, Adrb1 antibody, adrb1.L antibody, adrb1 antibody

Background: ADRB1, adrenergic, beta-1-, receptor, ADRB1R, B1AR, BETA1AR, RHR, beta-1-adrenergic receptor

Molecular Weight: 51.2kDa

Gene ID: 153

NCBI Accession: NP_000675

Pathways: cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Brown Fat Cell Differentiation