AGO1 antibody (Internal Region)

Catalog No. ABIN185581

Product Details anti-AGO1 Antibody

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: EIF2C1 / AGO1

Sequence: KNASYNLDPY IQEF

Specificity: This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Human EIF2C1bands of approx. 110 kDa and 35 kDa are observed. The 110 kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 kDa according to NP_036331.1. Recom

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AGO1

Target: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Alternative Name: AGO1 (EIF2C1 Products)

Background: EIF2C1, eukaryotic translation initiation factor 2C, 1, AGO1, EIF2C, GERP95, Q99 , Golgi Endoplasmic Reticulum protein 95 kDa, argonaute 1, putative RNA-binding protein Q99

Gene ID: 26523

NCBI Accession: NP_036331

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization