Stathmin 1 antibody (C-Term)

Catalog No. ABIN185573

Product Details anti-Stathmin 1 Antibody

Target: Stathmin 1 (STMN1)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Stathmin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Enzyme Immunoassay (EIA)

Purpose: STMN1 / Stathmin 1

Sequence: KNKESKDPAD ETEAD

Specificity: This antibody is expected to cross-react with isoform 1 a (NP_005554.1, NP_981944.1, NP_981946.1) only. The three reported isoforms (NP_005554.1, NP_981944.1 and NP_981946.1) represent identical protein.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KNKESKDPADETEAD, from the C Terminus of the protein sequence according to NP_005554.1, NP_981944.1, NP_981946.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus and Tonsil. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 17 kDa band observed in Human Testis and Mouse Brain lysates (calculated MW of 17.3 kDa according to NP_005554.1, NP_981944.1 and NP_981946.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

ELISA: This antibody has been successfully used in ELISA as described in the following paper: Song et al, Aging Cell. 2010 Aug,9(4):607-15, PMID: 20560902.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Stathmin 1 antibody (ABIN185573)

Watabe-Rudolph, Song, Lausser, Schnack, Begus-Nahrmann, Scheithauer, Rettinger, Otto, Tumani, Thal, Attems, Jellinger, Kestler, von Arnim, Rudolph: "Chitinase enzyme activity in CSF is a powerful biomarker of Alzheimer disease." in: Neurology, Vol. 78, Issue 8, pp. 569-77, (2012) (PubMed).

Song, von Figura, Liu, Kraus, Torrice, Dillon, Rudolph-Watabe, Ju, Kestler, Sanoff, Lenhard Rudolph: "Lifestyle impacts on the aging-associated expression of biomarkers of DNA damage and telomere dysfunction in human blood." in: Aging cell, Vol. 9, Issue 4, pp. 607-15, (2010) (PubMed).

Target Details for Stathmin 1

Target: Stathmin 1 (STMN1)

Alternative Name: STMN1 (STMN1 Products)

Background: STMN1, stathmin 1/oncoprotein 18 , LAP18, Lag, OP18, PP17, PP19, PR22, SMN , Pr22 protein, leukemia-associated phosphoprotein p18, metablastin, oncoprotein 18, phosphoprotein 19, prosolin, stathmin 1, stathmin 1 variant

Gene ID: 3925

NCBI Accession: NP_005554, NP_981944, NP_981946

Pathways: MAPK Signaling, Microtubule Dynamics