ARHGEF5 antibody (Internal Region)

Catalog No. ABIN185572

Product Details anti-ARHGEF5 Antibody

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARHGEF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: GEF5

Sequence: DKEIDQNSQQ EE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DKEIDQNSQQEE, from the internal region of the protein sequence according to NP_005426.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 170 kDa band observed in Human HeLa lysates (calculated MW of 177 kDa according to NP_005426.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARHGEF5

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Alternative Name: ARHGEF5 (ARHGEF5 Products)

Synonyms: ARHGEF5 antibody, GEF5 antibody, P60 antibody, TIM antibody, TIM1 antibody, 2210412D05Rik antibody, AA717842 antibody, AW495314 antibody, Tim1 antibody, Rho guanine nucleotide exchange factor 5 antibody, rho guanine nucleotide exchange factor 5 antibody, Rho guanine nucleotide exchange factor (GEF) 5 antibody, ARHGEF5 antibody, LOC100414419 antibody, Arhgef5 antibody

Background: GEF5, DKFZp686N1969, ARHGEF5, Rho guanine nucleotide exchange factor (GEF) 5, P60, TIM, TIM1, guanine nucleotide regulatory protein TIM, oncogene TIM rho guanine nucleotide exchange factor 5, transforming immortalized mammary oncogene

Gene ID: 7984

NCBI Accession: NP_005426