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CD14 antibody (Internal Region)

Name: CD14 antibody (Internal Region)
Brand: antibodies-online
SKU: ABIN185563
Price: 469.28 USD
Availability: InStock

Verified CD14 Reactivity: Human FACS, WB, ELISA Host: Goat Polyclonal unconjugated

(ABIN185563) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0. (CD14 antibody (Internal Region))

FACS

(ABIN185563) (2 μg/mL) staining of Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysate (35 μg protein in RIPA buffer) Detected by chemiluminescence. (CD14 antibody (Internal Region))

ABIN185563 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). (CD14 antibody (Internal Region))

ABIN185563 (1µg/ml) staining of PBM lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. (CD14 antibody (Internal Region))

ABIN185563 (4µg/ml) staining of paraffin embedded Human Spleen. (CD14 antibody (Internal Region))

IHC

5 images

Catalog No. ABIN185563

Datasheet as PDF

Target See all CD14 Antibodies

CD14
Binding Specificity
9

6

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reactivity
231

38

37

25

25

23

23

23

18

16

15

15

14

14

13

10

8

8

6

5

5

2

1

1
Human
Host
206

52

16

2

1
Goat
Compatible Secondaries
Clonality
224

53
Polyclonal
Conjugate
115

33

23

19

13

5

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD14 antibody is un-conjugated
Application
209

81

61

50

49

45

21

15

13

11

7

4

4

3

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA

Purpose

CD14

Sequence

KRVDADADPR QYAD

Cross-Reactivity

Human

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1.

Isotype

IgG

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Application Notes

Western Blot: Approx 55 kDa band observed in Human Lymph node and Tonsil lysates (calculated MW of 40.1 kDa according to NP_000582.1).The observed molecular weight corresponds to the glycosylated form, and was successfully blocked by incubation with the im
Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

CD14

Alternative Name

CD14 (CD14 Products)

Synonyms

CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background

CD14, CD14 antigen , HGNC:1628

Gene ID

929

NCBI Accession

NP_000582

Pathways

TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades