IFT88 antibody (C-Term)

Catalog No. ABIN185555

Product Details anti-IFT88 Antibody

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT88 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: IFT88 / Polaris

Sequence: KKRIDEDDFA DEE

Specificity: This antibody is expected to recognise many reported isoforms.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IFT88 antibody (ABIN185555)

Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." in: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

Target Details for IFT88

Target: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Alternative Name: IFT88 (IFT88 Products)

Synonyms: 146590_s_at antibody, CG12548 antibody, CG14465 antibody, CG15190 antibody, CG4137 antibody, Dmel\\CG12548 antibody, IFT88 antibody, NOMPB antibody, OSM-5 antibody, Tg737 antibody, nomp antibody, osm-5 antibody, AW552028 antibody, Tg737Rpw antibody, TgN737Rpw antibody, Ttc10 antibody, flexo antibody, fxo antibody, orpk antibody, polaris antibody, D13S1056E antibody, DAF19 antibody, TG737 antibody, TTC10 antibody, hTg737 antibody, intraflagellar transport 88 antibody, intraflagellar transport 88 homolog antibody, intraflagellar transport protein 88 homolog antibody, no mechanoreceptor potential B antibody, IFT88 antibody, ift88 antibody, LOC100518926 antibody, LOC100640298 antibody, LOC100646898 antibody, nompB antibody, Ift88 antibody

Background: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra

Gene ID: 8100, 21821, 305918

NCBI Accession: NP_783195, NP_006522, NP_001340507, NP_001340498

Pathways: Hedgehog Signaling, Sensory Perception of Sound