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VPS25 antibody (Internal Region)

Verified VPS25 Reactivity: Human, Rat WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185544
  • Target See all VPS25 Antibodies
    VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
    Binding Specificity
    • 14
    • 8
    • 7
    • 1
    • 1
    Internal Region
    Reactivity
    • 27
    • 19
    • 4
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 31
    • 2
    Goat
    Clonality
    • 32
    • 2
    Polyclonal
    Conjugate
    • 14
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This VPS25 antibody is un-conjugated
    Application
    • 23
    • 22
    • 5
    • 4
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    VPS25
    Sequence
    QPNVDTRQKQ
    Cross-Reactivity
    Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-QPNVDTRQKQ, from the internal region of the protein sequence according to NP_115729.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx. 22 kDa band observed in Rat Kidney lysates (calculated MW of 20.7 kDa according to human NP_115729.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))
    Alternative Name
    VPS25 (VPS25 Products)
    Synonyms
    VPS25 antibody, CG14750 antibody, Dmel\\CG14750 antibody, ESCRT-I antibody, ESCRT-II antibody, dVps25 antibody, l(2)44Db antibody, l(2)k08904 antibody, vps25 antibody, GB16268 antibody, derp9 antibody, eap20 antibody, fap20 antibody, DDBDRAFT_0189483 antibody, DDBDRAFT_0234026 antibody, DDB_0189483 antibody, DDB_0234026 antibody, 1110020N13Rik antibody, AW107467 antibody, D11Wsu68e antibody, im:6898134 antibody, zgc:162560 antibody, DERP9 antibody, EAP20 antibody, FAP20 antibody, vacuolar protein sorting 25 homolog antibody, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene antibody, vacuolar protein sorting-associated protein 25 antibody, WNK lysine deficient protein kinase 4 antibody, Vacuolar protein sorting 25 antibody, vacuolar protein-sorting-associated protein 25 antibody, vacuolar protein sorting 25 homolog L homeolog antibody, vacuolar protein sorting 25 antibody, vacuolar protein sorting 25 homolog (S. cerevisiae) antibody, E2F/DP family winged-helix DNA-binding domain-containing protein antibody, VPS25 antibody, LOC459431 antibody, CpipJ_CPIJ010292 antibody, WNK4 antibody, Vps25 antibody, LOC412381 antibody, vps25.L antibody, vps25 antibody
    Background
    VPS25, vacuolar protein sorting 25 (yeast), HGNC:28122, DERP9, EAP20, MGC10540, vacuolar protein sorting 25, vacuolar protein sorting 25 homolog (S. cerevisiae), FAP20
    Gene ID
    84313
    NCBI Accession
    NP_115729
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