GAPDH antibody (Internal Region)

Catalog No. ABIN185543

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: GAPDH (Internal)

Sequence: GVNHEKYDNS LK

Specificity: This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1 kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-GAPDH antibody (ABIN185543)

Ramsden, Nommiste, R Lane, Carr, Powner, J K Smart, Chen, Muthiah, Webster, Moore, Cheetham, da Cruz, Coffey: "Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs." in: Scientific reports, Vol. 7, Issue 1, pp. 51, (2019) (PubMed).

Carr, Vugler, Yu, Semo, Coffey, Moss, Greenwood: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." in: Molecular vision, Vol. 17, pp. 1701-15, (2011) (PubMed).

Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." in: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." in: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." in: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." in: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

Gene ID: 2597, 14433, 24383

NCBI Accession: NP_002037, NP_001243728