PPIA antibody (Internal Region)
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- Target See all PPIA Antibodies
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PPIA antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- Cyclophilin A / PPIA
- Sequence
- ERFGSRNGKT SKK
- Specificity
- This product is expected to also recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-ERFGSRNGKTSKK, from the internal region of the protein sequence according to NP_066953.1.
- Isotype
- IgG
- Top Product
- Discover our top product PPIA Primary Antibody
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- Application Notes
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Western Blot: Approx.18 kDa band observed in Human Lymph Node lysates (calculated MW 18.0 kDa according to NP_066953.1). Recommended Concetration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:8000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
- Alternative Name
- PPIA (PPIA Products)
- Background
- PPIA, peptidylprolyl isomerase A (cyclophilin A) , HGNC:9253, CYPA, CYPH, MGC12404 , T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase, MGC117158, MGC23397, PPIase A
- Gene ID
- 5478
- NCBI Accession
- NP_066953
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