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KPNB1 antibody (C-Term)

Verified KPNB1 Reactivity: Human, Rat WB, ELISA, IHC, FACS, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN185516
  • Target See all KPNB1 Antibodies
    KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
    Binding Specificity
    • 15
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 45
    • 18
    • 16
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 43
    • 2
    • 1
    Goat
    Clonality
    • 43
    • 3
    Polyclonal
    Conjugate
    • 22
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KPNB1 antibody is un-conjugated
    Application
    • 34
    • 13
    • 13
    • 10
    • 9
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    Karyopherin (importin) beta 1
    Sequence
    RRSKTNKAKT LAT
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 90-95- kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2 kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incu

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))
    Alternative Name
    KPNB1 (KPNB1 Products)
    Synonyms
    Afu1g15720 antibody, AO090005001133 antibody, imb1 antibody, impnb antibody, ipo1 antibody, ipob antibody, ntf97 antibody, IMB1 antibody, IPO1 antibody, IPOB antibody, Impnb antibody, NTF97 antibody, AA409963 antibody, importin beta-1 subunit antibody, Importin beta-1 subunit antibody, karyopherin (importin) beta 1 L homeolog antibody, importin beta 1 antibody, karyopherin subunit beta 1 antibody, karyopherin (importin) beta 1 antibody, AFUA_1G15720 antibody, AOR_1_1974174 antibody, Bm1_57540 antibody, PMAA_065830 antibody, AFLA_083750 antibody, TSTA_085870 antibody, BDBG_07509 antibody, MCYG_00039 antibody, kpnb1.L antibody, LOC100533387 antibody, KPNB1 antibody, Kpnb1 antibody
    Background
    KPNB1, karyopherin (importin) beta 1, HGNC:6400, IMB1, IPOB, Impnb, MGC2155, MGC2156, MGC2157, NTF97, importin 90, importin beta-1 subunit, karyopherin beta 1, nuclear factor p97, KapB1, IPO1
    Gene ID
    3837, 16211, 24917
    NCBI Accession
    NP_002256
    Pathways
    Protein targeting to Nucleus
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