CBX3 antibody (Internal Region)

Catalog No. ABIN185493

Product Details anti-CBX3 Antibody

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: CBX3 / HP1 Gamma

Sequence: EAFLNSQKAG KEKD

Specificity: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2, NP_057671.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx. 20 kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8 kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-CBX3 antibody (ABIN185493)

Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: CBX3 (CBX3 Products)

Synonyms: HECH antibody, HP1-GAMMA antibody, HP1Hs-gamma antibody, Hp1-gamma antibody, HP1g antibody, M32 antibody, cbx3 antibody, cbx3a antibody, xhp1g antibody, wu:fb61b02 antibody, zgc:153397 antibody, chromobox 3 antibody, chromobox 3 L homeolog antibody, chromobox protein homolog 3 pseudogene antibody, chromobox homolog 3a (HP1 gamma homolog, Drosophila) antibody, CBX3 antibody, Cbx3 antibody, cbx3.L antibody, LOC100355282 antibody, cbx3a antibody

Background: CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila) , HGNC:1553, HECH, HP1Hs-gamma , HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1

Gene ID: 11335, 12417, 297093

NCBI Accession: NP_009207