CBX5 antibody (Internal Region)

Catalog No. ABIN185491

Product Details anti-CBX5 Antibody

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: CBX5 / HP1-Alpha

Sequence: NKRKSNFSNS ADDIK

Specificity: Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NKRKSNFSNSADDIK, from the internal region of the protein sequence according to NP_036249.1, NP_001120793.1, NP_001120794.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 24-25 kDa band observed in lysates of cell lines A43N and K562 (calculated MW of 22.2 kDa according to Human NP_036249.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: CBX5 (CBX5 Products)

Synonyms: HP1 antibody, HP1A antibody, id:ibd2026 antibody, wu:fc63e02 antibody, wu:fe47g12 antibody, wu:fi03h05 antibody, zgc:158601 antibody, CBX5 antibody, cbx5 antibody, MGC64354 antibody, 2610029O15Rik antibody, C75991 antibody, Hp1a antibody, Hp1alpha antibody, chromobox 5 antibody, chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody, chromobox 5 S homeolog antibody, CBX5 antibody, cbx5 antibody, Cbx5 antibody, cbx5.S antibody

Background: CBX5, HP1-ALPHA, chromobox homolog 5 (HP1 alpha homolog, Drosophila), HGNC:1555, HP1, HP1Hs-alpha , chromobox homolog 5 (Drosophila HP1 alpha), Heterochromatin protein-1, HP1A, antigen p25, heterochromatin protein 1 homolog alpha, heterochromatin protein

Gene ID: 23468

NCBI Accession: NP_036249, NP_001120793, NP_001120794