ENPP1 antibody (C-Term)

Catalog No. ABIN185487

Product Details anti-ENPP1 Antibody

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPP1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: ENPP1 / PC1

Sequence: KTHLPTFSQE D

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thyroid Gland. Recommended concentration: 4 μg/mL.

Western Blot: Preliminary testing showed bands at approx., 25+150 kDa in Human Liver lysates after 0.5-1 μg/mL antibody staining (calculated Mwt of 105 kDa according to NP_006199.2). Both bands were successfully blocked by incubation with the immunizing pep

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ENPP1 antibody (ABIN185487)

Lau, Doucet, Stadel, Huang, Weber, Kominsky: "Enpp1: a potential facilitator of breast cancer bone metastasis." in: PLoS ONE, Vol. 8, Issue 7, pp. e66752, (2013) (PubMed).

Simão, Yadav, Narisawa, Bolean, Pizauro, Hoylaerts, Ciancaglini, Millán: "Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7598-609, (2010) (PubMed).

Target Details for ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Alternative Name: ENPP1 (ENPP1 Products)

Synonyms: ENPP1 antibody, si:ch211-142e24.2 antibody, zgc:136238 antibody, ARHR2 antibody, M6S1 antibody, NPP1 antibody, NPPS antibody, PC-1 antibody, PCA1 antibody, PDNP1 antibody, 4833416E15Rik antibody, AI428932 antibody, C76301 antibody, CD203c antibody, E-NPP1 antibody, Ly-41 antibody, Npps antibody, Pca antibody, Pca-1 antibody, Pdnp1 antibody, ttw antibody, twy antibody, Pc1 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 1 L homeolog antibody, ENPP1 antibody, enpp1 antibody, enpp1.L antibody, Enpp1 antibody

Background: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide

Gene ID: 5167

NCBI Accession: NP_006199

Pathways: Regulation of Carbohydrate Metabolic Process