Nanog antibody (Internal Region)

Catalog No. ABIN185444

Product Details anti-Nanog Antibody

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: NANOG

Sequence: QNQRMKSKRW QKNN

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1,21(15):3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22,7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012,21(

Western Blot: Approx 38 kDa band observed in Human Ovary lysates (calculated MW of 34.6 kDa according to NP_079141.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human: PMID: 30878013, 21681858, 22613719, 21877920. 21285354,19890879, 19483

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Nanog antibody (ABIN185444)

Sancho-Martinez, Nivet, Xia, Hishida, Aguirre, Ocampo, Ma, Morey, Krause, Zembrzycki, Ansorge, Vazquez-Ferrer, Dubova, Reddy, Lam, Hishida, Wu, Esteban, OLeary, Wahl, Verma, Laurent, Izpisua Belmonte: "Establishment of human iPSC-based models for the study and targeting of glioma initiating cells." in: Nature communications, Vol. 7, pp. 10743, (2016) (PubMed).

Tolar, McGrath, Xia, Riddle, Lees, Eide, Keene, Liu, Osborn, Lund, Blazar, Wagner: "Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa." in: The Journal of investigative dermatology, Vol. 134, Issue 5, pp. 1246-54, (2014) (PubMed).

Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).

Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).

Montserrat, de Oñate, Garreta, González, Adamo, Eguizábal, Häfner, Vassena, Izpisua Belmonte: "Generation of feeder-free pig induced pluripotent stem cells without Pou5f1." in: Cell transplantation, Vol. 21, Issue 5, pp. 815-25, (2012) (PubMed).

Montserrat, Garreta, González, Gutiérrez, Eguizábal, Ramos, Borrós, Izpisua Belmonte: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).

Montserrat, Bahima, Batlle, Häfner, Rodrigues, González, Izpisúa Belmonte: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).

Eguizabal, Montserrat, Vassena, Barragan, Garreta, Garcia-Quevedo, Vidal, Giorgetti, Veiga, Izpisua Belmonte: "Complete meiosis from human induced pluripotent stem cells." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 8, pp. 1186-95, (2011) (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena, González, Barrero, Veiga, Raya, Izpisúa Belmonte: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).

Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: NANOG (NANOG Products)

Synonyms: 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

Background: NANOG, Nanog homeobox, HGNC:20857 , homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Gene ID: 79923, 71950, 414065

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance