HMGA1 antibody (Internal Region)

Catalog No. ABIN185443

Product Details anti-HMGA1 Antibody

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: HMGA1

Sequence: LASKQEKDGT EK

Specificity: This antibody is expected to recognise isoform a (also called HMG-I, NP_665906.1, NP_665908.1) and isoform b (also called HMG-Y, NP_002122.1, NP_665909.1, NP_665910.1, NP_665912.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-LASKQEKDGTEK, from the internal region of the protein sequence according to NP_665906.1, NP_665908.1, NP_002122.1, NP_665909.1, NP_665912.1, NP_665910.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Small Intestine. Recommended concentration: 4 μg/mL.

Western Blot: Preliminary experiments gave an approx 50 kDa band in Human Testis and Ovary lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in qRT-PCR on Human: Cello et al. (2013) PMID: 23545254.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HMGA1 antibody (ABIN185443)

Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

Target Details for HMGA1

Target: HMGA1 (High Mobility Group AT-Hook 1 (HMGA1))

Alternative Name: HMGA1 (HMGA1 Products)

Background: HMGA1, high mobility group AT-hook 1 , HGNC:5010, HMG-R, HMGIY, MGC12816, MGC4242, MGC4854 , OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, HMGA1A,

Gene ID: 3159

NCBI Accession: NP_665906, NP_665908, NP_002122, NP_665909, NP_665912, NP_665910

Pathways: Nuclear Hormone Receptor Binding, Autophagy