LRP5 antibody (Internal Region)

Catalog No. ABIN185410

Product Details anti-LRP5 Antibody

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LRP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: LRP5

Sequence: ERVEKTTGDK RT

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ERVEKTTGDKRT, from the internal region of the protein sequence according to NP_002326.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows weak cytoplasm staining in some hepatocytes. Recommended concentration: 2-4 μg/mL.

Western Blot: Approx 180 kDa band observed in lysates of cell line HepG2 (calculated MW of 179 kDa according to NP_002326.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver for this p

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-LRP5 antibody (ABIN185410)

Dong, Li, Li, Ren, Fan, Weng: "MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 9331-9336, (2018) (PubMed).

Target Details for LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP5 (LRP5 Products)

Background: LRP5, low density lipoprotein receptor-related protein 5, HGNC:6697, BMND1, HBM, LR3, LRP7, OPPG, OPS, VBCH2, low density lipoprotein receptor-related protein 7, osteoporosis pseudoglioma syndrome, EVR1, EVR4, OPTA1, exudative vitreoretinopathy 1,

Gene ID: 4041

NCBI Accession: NP_002326

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation