ADAM33 antibody (Internal Region)

Catalog No. ABIN185401

Product Details anti-ADAM33 Antibody

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAM33 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ADAM33

Sequence: CQSRRCRKNA FQEL

Specificity: This antibody is expected to recognise isoform alpha (NP_079496.1) and not isoform beta (NP_694882.1).

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CQSRRCRKNAFQEL, from the internal region of the protein sequence according to NP_079496.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Experiments gave bands at approx 80-90 kDa and strong ladder from 26 to 38 kDa in Human Prostate lysates after 0.3 μg/mL antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul,31(1):13-21, PMID: 14742294). This protein has a calculated MW of 87.7 kDa according to NP_079496.1. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADAM33

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Alternative Name: ADAM33 (ADAM33 Products)

Synonyms: ADAM13 antibody, ADAM33 antibody, Adaml antibody, adam13 antibody, C20orf153 antibody, DJ964F7.1 antibody, ADAM metallopeptidase domain 33 antibody, disintegrin and metalloproteinase domain-containing protein 19-like antibody, a disintegrin and metallopeptidase domain 33 antibody, ADAM metallopeptidase domain 33 L homeolog antibody, ADAM33 antibody, LOC100230755 antibody, Adam33 antibody, adam33.L antibody

Background: ADAM33, a disintegrin and metalloproteinase domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, a disintegrin and metalloprotease 33, disintegrin and reprolysin metalloproteinase family protein, metalloprotease disintegrin, ADAM metallopeptidase domain 33, RP5-964F7.2, C20orf153, MGC149823

Gene ID: 80332

NCBI Accession: NP_079496