PAI1 antibody (Internal Region)

Catalog No. ABIN185384

Product Details anti-PAI1 Antibody

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: PAI1 / SERPINE1

Sequence: GFKIDDKGMA PALRH

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1, NP_001158885.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6 μg/mL. A customer successfully used this product on Human Breast cancer.

Western Blot: Approx 48 kDa band observed in Human Liver and HeLa lysates, while approx. 45 kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1 kDa according to NP_000593.1). This molecular weight is routinely observed by other source

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PAI1 antibody (ABIN185384)

Kozaczuk, Selmi, Bednarek: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." in: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, (2013) (PubMed).

Target Details for PAI1

Target: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Alternative Name: SERPINE1 (SERPINE1 Products)

Background: SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator in

Molecular Weight: 45.1kDa

Gene ID: 5054

NCBI Accession: NP_000593

Pathways: p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration