NPM1 antibody (C-Term)

Catalog No. ABIN185372

Product Details anti-NPM1 Antibody

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purpose: Nucleophosmin / NPM1

Sequence: QEAIQDLWQW RKSL

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6 kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed b

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1 (NPM1 Products)

Background: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1

Gene ID: 4869

NCBI Accession: NP_001341935, NP_954654, NP_001341936, NP_001341939

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly