MYD88 antibody (Internal Region)

Catalog No. ABIN185362

Product Details anti-MYD88 Antibody

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MYD88 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: MYD88

Sequence: IKYKAMKKEF P

Specificity: This antibody is expected to recognize reported isoforms 1, 2, 3 and 9.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_002459.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Spleen lysates (calculated MW of 28.3 kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5 μg/mL. Primary incubation1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Alternative Name: MYD88 (MYD88 Products)

Background: MYD88, myeloid differentiation primary response gene (88), MYD88D, myeloid differentiation primary response gene 88

Molecular Weight: 33.2kDa

Gene ID: 4615, 17874, 301059

NCBI Accession: NP_001166038, NP_002459, NP_001166039

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades