TPI1 antibody (C-Term)

Catalog No. ABIN185349

Product Details anti-TPI1 Antibody

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Triosephosphate isomerase

Sequence: LKPEFVDIIN AKQ

Specificity: This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1, NP_001152759.1, NP_001244955.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7 kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TPI1 antibody (ABIN185349)

Honoré, Buus, Claësson: "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).

Vorum, Østergaard, Rice, Honoré, Bek: "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study." in: Proteome science, Vol. 5, pp. 5, (2007) (PubMed).

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: TPI1 (TPI1 Products)

Background: TPI1, triosephosphate isomerase 1, TPI, MGC88108

Gene ID: 7167, 21991, 24849

NCBI Accession: NP_000356, NP_001152759

Pathways: Cell RedoxHomeostasis