TPI1 antibody (C-Term)

Catalog No. ABIN185349

Product Details anti-TPI1 Antibody

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Triosephosphate isomerase

Sequence: LKPEFVDIIN AKQ

Specificity: This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1, NP_001152759.1, NP_001244955.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows staining of the sinusoids. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Pancreas. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in lysates of cell lines HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK (calculated MW of 26.7 kDa according to Human NP_000356.1). Recommended concentration: 0.001-0.01 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TPI1 antibody (ABIN185349)

Honoré, Buus, Claësson: "Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53." in: Proteome science, Vol. 6, pp. 18, (2008) (PubMed).

Vorum, Østergaard, Rice, Honoré, Bek: "Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis--a proteomic study." in: Proteome science, Vol. 5, pp. 5, (2007) (PubMed).

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: TPI1 (TPI1 Products)

Synonyms: TIM antibody, TPI antibody, TIM1 antibody, hTIM antibody, tpi antibody, tpi1 antibody, TPI1 antibody, AI255506 antibody, Tpi antibody, Tpi-1 antibody, GB17473 antibody, Tim antibody, CG2171 antibody, Dmel\\CG2171 antibody, ND14 antibody, TPIS antibody, sgk antibody, wstd antibody, DrTPI-A antibody, DrTPI-B antibody, TIM-B antibody, cb179 antibody, wu:fb51c02 antibody, triosephosphate isomerase 1 antibody, timeless circadian regulator antibody, triosephosphate isomerase 1 S homeolog antibody, triosephosphate isomerase antibody, triose-phosphate isomerase antibody, Triose-phosphate isomerase antibody, triose-phosphate isomerase, putative antibody, timeless circadian clock antibody, Triose phosphate isomerase antibody, Triosephosphate isomerase antibody, triosephosphate isomerase 1a antibody, triosephosphate isomerase 1b antibody, TPI1 antibody, TIMELESS antibody, tpi1 antibody, Tpi1 antibody, tpi1.S antibody, Tpi antibody, tpiA antibody, CNE05000 antibody, Fnod_0738 antibody, Tlet_0996 antibody, SARE_RS16215 antibody, PKH_126640 antibody, Timeless antibody, tpi-1 antibody, tpi1a antibody, tpi1b antibody

Background: TPI1, triosephosphate isomerase 1, TPI, MGC88108

Gene ID: 7167, 21991, 24849

NCBI Accession: NP_000356, NP_001152759

Pathways: Cell RedoxHomeostasis