HSD17B10 antibody (C-Term)

Catalog No. ABIN185330

Product Details anti-HSD17B10 Antibody

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HSD17B10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: ABAD / HADH2

Sequence: CIRLDGAIRM QP

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CIRLDGAIRMQP, from the C Terminus of the protein sequence according to NP_004484.1, NP_001032900.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Human Brain lysates (calculated MW of 26.9 kDa according to NP_004484). Recommended concentration: 0.3-2 μg/mL. Primary incubation 1 hour at room temperature. A minor band of unknown identity was also consistent

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HSD17B10 antibody (ABIN185330)

Tilton, Haidacher, Lejeune, Zhang, Zhao, Kurosky, Brasier, Denner: "Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry." in: Proteomics, Vol. 7, Issue 10, pp. 1729-42, (2007) (PubMed).

Target Details for HSD17B10

Target: HSD17B10 (Hydroxysteroid (17-Beta) Dehydrogenase 10 (HSD17B10))

Alternative Name: HSD17B10 (HSD17B10 Products)

Background: HSD17B10, HADH2, ABAD, ERAB, MHBD, 17b-HSD10, hydroxyacyl-Coenzyme A dehydrogenase, type II, type 10 17b-HSD, AB-binding alcohol dehydrogenase, type 10 17beta-hydroxysteroid dehydrogenase, hydroxysteroid (17-beta) dehydrogenase 10, RP3-339A18.2, 17b-HSD10

Gene ID: 3028

NCBI Accession: NP_004484, NP_001032900