VAV3 antibody (Internal Region)

Catalog No. ABIN185278

Product Details anti-VAV3 Antibody

Target: VAV3 (Vav 3 Oncogene (VAV3))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: VAV3

Sequence: C-SGEQGTLKLP EK

Specificity: This antibody is expected to recognize both reported isoforms (NP_006104.4, NP_001073343.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CSGEQGTLKLPEK, from the internal region of the protein sequence according to NP_006104.4, NP_001073343.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 7.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: VAV3 (VAV3 Products)

Synonyms: RGD1565941 antibody, A530094I06Rik antibody, AA986410 antibody, Idd18.1 antibody, vav guanine nucleotide exchange factor 3 antibody, vav 3 guanine nucleotide exchange factor antibody, vav 3 oncogene antibody, VAV3 antibody, Vav3 antibody

Background: VAV3, vav 3 oncogene, vav 3 guanine nucleotide exchange factor, FLJ40431

Gene ID: 10451

NCBI Accession: NP_006104, NP_001073343

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway