Survivin antibody (C-Term)

Catalog No. ABIN185270

Product Details anti-Survivin Antibody

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Survivin / BIRC5

Sequence: CVRRAIEQLA AMD

Specificity: This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1).

Cross-Reactivity: Cat, Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CVRRAIEQLAAMD, from the C Terminus of the protein sequence according to NP_001159.2, NP_001012271.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 18 kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4 kDa according to NP_001159.2 and 18.6 kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: BIRC5 (BIRC5 Products)

Background: BIRC5, survivin, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), apoptosis inhibitor 4, baculoviral IAP repeat-containing 5, baculoviral IAP repeat-containing protein 5, survivin variant 3 alpha

Gene ID: 332

NCBI Accession: NP_001159, NP_001012271

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding