FTCD antibody (N-Term)

Catalog No. ABIN185267

Product Details anti-FTCD Antibody

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FTCD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: 58K Golgi protein(N-Term)/FTCD

Sequence: SQLVECVPNF SEGKNQ

Specificity: Reported variants represent identical protein: NP_996848.1, NP_006648.1

Cross-Reactivity: Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence SQLVECVPNFSEGKNQ, from the N Terminus of the protein sequence according to NP_006648.1, NP_996848.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 58-60 kDa band observed in Human Liver lysates and in lysates of cell line HepG2 (calculated MW of 58.9 kDa according to NP_006648.1). Recommended concentration: 0.05-0.1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FTCD antibody (ABIN185267)

Hagiwara, Tajika, Matsuzaki, Suzuki, Aoki, Takata: "Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome." in: Histochemistry and cell biology, Vol. 126, Issue 2, pp. 251-9, (2006) (PubMed).

Target Details for FTCD

Target: FTCD (Formiminotransferase Cyclodeaminase (FTCD))

Alternative Name: FTCD (FTCD Products)

Synonyms: LCHC1 antibody, FTCD antibody, wu:fp37b11 antibody, zgc:63647 antibody, formimidoyltransferase cyclodeaminase antibody, formiminotransferase cyclodeaminase antibody, formiminotransferase-cyclodeaminase antibody, formimidoyltransferase cyclodeaminase L homeolog antibody, FTCD antibody, Ftcd antibody, ftcd antibody, DP2350 antibody, Cbei_0990 antibody, TRQ2_1223 antibody, GAU_0899 antibody, ftcd.L antibody

Background: FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase

Gene ID: 10841, 14317

NCBI Accession: NP_006648