XAGE1B/GAGED2 antibody (C-Term)
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- Target See all XAGE1B/GAGED2 (XAGE1B) Antibodies
- XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))
- Binding Specificity
- C-Term
- Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This XAGE1B/GAGED2 antibody is un-conjugated
- Application
- ELISA, Immunohistochemistry (IHC)
- Purpose
- XAGE1 (Isoform 1d) / GAGED2
- Sequence
- CGFGFRRQGE DNT
- Specificity
- This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1, NP_001091067.1, NP_001091074.1, NP_001091062.1, NP_001091065.1).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- CGFGFRRQGEDNT
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6 μg/mL.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0 kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the produ
Peptide ELISA: antibody detection limit dilution 1:8000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))
- Alternative Name
- GAGED2 (XAGE1B Products)
- Synonyms
- CT12.1 antibody, CT12.1B antibody, CTP9 antibody, GAGED2 antibody, XAGE-1 antibody, X antigen family member 1B antibody, XAGE1B antibody
- Background
- GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAGE-1 protein
- Gene ID
- 9503
- NCBI Accession
- NP_597673
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