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XAGE1B/GAGED2 antibody (C-Term)

Verified XAGE1B Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185262
  • Target See all XAGE1B/GAGED2 (XAGE1B) Antibodies
    XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))
    Binding Specificity
    C-Term
    Reactivity
    Human
    Host
    • 1
    Goat
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This XAGE1B/GAGED2 antibody is un-conjugated
    Application
    ELISA, Immunohistochemistry (IHC)
    Purpose
    XAGE1 (Isoform 1d) / GAGED2
    Sequence
    CGFGFRRQGE DNT
    Specificity
    This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1, NP_001091067.1, NP_001091074.1, NP_001091062.1, NP_001091065.1).
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    CGFGFRRQGEDNT
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6 μg/mL.

    Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0 kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the produ

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    XAGE1B/GAGED2 (XAGE1B) (X Antigen Family, Member 1B (XAGE1B))
    Alternative Name
    GAGED2 (XAGE1B Products)
    Synonyms
    CT12.1 antibody, CT12.1B antibody, CTP9 antibody, GAGED2 antibody, XAGE-1 antibody, X antigen family member 1B antibody, XAGE1B antibody
    Background
    GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAGE-1 protein
    Gene ID
    9503
    NCBI Accession
    NP_597673
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