RANBP1 antibody (C-Term)

Catalog No. ABIN185257

Product Details anti-RANBP1 Antibody

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RANBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: RANBP1

Sequence: LSVKEETKED AEEKQ

Specificity: This antibody is expected to recognise reported isoforms 1, 2, 3 and 4.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-LSVKEETKEDAEEKQ

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows pixulated cytoplasm staining in DCT. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 26-27 kDa band observed in lysates of cell lines A431 HeLa and MCF7 and in Human Duodenum lysates, (calculated MW of 23.3 kDa according to NP_002873.1). This molecular weight is routinely observed by other sources. Recommended concent

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentrati

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RANBP1

Target: RANBP1 (RAN Binding Protein 1 (RANBP1))

Alternative Name: RANBP1 (RANBP1 Products)

Synonyms: MGC64314 antibody, zeh1094 antibody, zgc:85649 antibody, hm:zeh1094 antibody, wu:fa96a09 antibody, wu:fj39g07 antibody, HTF9A antibody, Htf9a antibody, RAN binding protein 1 S homeolog antibody, RAN binding protein 1 L homeolog antibody, ran binding protein 1 antibody, RAN binding protein 1 antibody, ran-specific GTPase-activating protein pseudogene antibody, ranbp1.S antibody, ranbp1.L antibody, PVX_090070 antibody, GL50803_16969 antibody, LOC100343953 antibody, ranbp1 antibody, RANBP1 antibody, Ranbp1 antibody

Background: RANBP1, RAN binding protein 1, HTF9A, MGC88701

Gene ID: 5902

NCBI Accession: NP_002873