ACTR1B antibody (C-Term)

Catalog No. ABIN185220

Product Details anti-ACTR1B Antibody

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTR1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: ARP1 homolog B

Sequence: YEEDGSRAIH RKT

Specificity: This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-YEEDGSRAIHRKT, from the C Terminus of the protein sequence according to NP_005726.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrial cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 45 kDa band observed in human heart and brain lysates (predicted MW of 43 kDa according to NP_005726). Recommended for use at 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Alternative Name: ACTR1B (ACTR1B Products)

Background: ACTR1B, ARP1B, CTRN2, ARP1 actin-related protein 1 homolog B, centractin beta (yeast), PC3, centractin beta, ARP1, yeast homolog B, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1 actin-related protein 1 homolog B, centractin beta

Gene ID: 10120, 226977

NCBI Accession: NP_005726