IFT74 antibody (C-Term)

Catalog No. ABIN185213

Product Details anti-IFT74 Antibody

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IFT74 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: CMG1 / CCDC2 / IFT74

Sequence: KTIVDALHST SGN

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2 kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IFT74 antibody (ABIN185213)

Boldt, Mans, Won, van Reeuwijk, Vogt, Kinkl, Letteboer, Hicks, Hurd, Naggert, Texier, den Hollander, Koenekoop, Bennett, Cremers, Gloeckner, Nishina, Roepman, Ueffing: "Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice." in: The Journal of clinical investigation, Vol. 121, Issue 6, pp. 2169-80, (2011) (PubMed).

Momeni, Schymick, Jain, Cookson, Cairns, Greggio, Greenway, Berger, Pickering-Brown, Chiò, Fung, Holtzman, Huey, Wassermann, Adamson, Hutton, Rogaeva, St George-Hyslop, Rothstein, Hardiman, Grafman et al.: "Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. ..." in: BMC neurology, Vol. 6, pp. 44, (2007) (PubMed).

Target Details for IFT74

Target: IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

Alternative Name: IFT74 (IFT74 Products)

Synonyms: CCDC2 antibody, DKFZp469F1522 antibody, 1700029H06Rik antibody, Ccdc2 antibody, Cmg1 antibody, b2b796Clo antibody, CMG-1 antibody, CMG1 antibody, intraflagellar transport protein 74/72 antibody, intraflagellar transport 74 antibody, intraflagellar transport protein 74 homolog antibody, IFT74 antibody, ift74 antibody, LOC100632007 antibody, LOC100649180 antibody, Ift74 antibody

Background: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

Gene ID: 80173, 313365

NCBI Accession: NP_001092692, NP_001092693, NP_079379

Pathways: Hedgehog Signaling, Chromatin Binding