NOXO1 antibody (C-Term)

Catalog No. ABIN185195

Product Details anti-NOXO1 Antibody

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOXO1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: NOXO1

Sequence: RGCVDSVPHP TTEQ

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence RGCVDSVPHPTTEQ, from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NOXO1 antibody (ABIN185195)

Wu, Gao, Ohlemeyer, Roos, Niessen, Köttgen, Gessner: "Activation of AP-1 through reactive oxygen species by angiotensin II in rat cardiomyocytes." in: Free radical biology & medicine, Vol. 39, Issue 12, pp. 1601-10, (2005) (PubMed).

Target Details for NOXO1

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Alternative Name: NOXO1 (NOXO1 Products)

Synonyms: NOXO1 antibody, cb18 antibody, sb:cb18 antibody, wu:fd09d09 antibody, noxo1 antibody, snx28 antibody, p41nox antibody, p41noxa antibody, p41noxb antibody, p41noxc antibody, sh3pxd5 antibody, P41NOX antibody, P41NOXA antibody, P41NOXB antibody, P41NOXC antibody, SH3PXD5 antibody, SNX28 antibody, 2310034C04Rik antibody, Snx28 antibody, hslt antibody, NADPH oxidase organizer 1 antibody, NOXO1 antibody, noxo1 antibody, noxo1.1 antibody, Noxo1 antibody

Background: NOXO1, P41NOX, P41NOXA, P41NOXB, P41NOXC, MGC20258, NADPH oxidase organizer 1, regulatory protein P41NOX, SH3PXD5, SNX28, Nox organizer 1

Gene ID: 124056

NCBI Accession: NP_653204, NP_751907