NOXO1 antibody (C-Term)

Catalog No. ABIN185195

Product Details anti-NOXO1 Antibody

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOXO1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: NOXO1

Sequence: RGCVDSVPHP TTEQ

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence RGCVDSVPHPTTEQ, from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NOXO1 antibody (ABIN185195)

Wu, Gao, Ohlemeyer, Roos, Niessen, Köttgen, Gessner: "Activation of AP-1 through reactive oxygen species by angiotensin II in rat cardiomyocytes." in: Free radical biology & medicine, Vol. 39, Issue 12, pp. 1601-10, (2005) (PubMed).

Target Details for NOXO1

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Alternative Name: NOXO1 (NOXO1 Products)

Background: NOXO1, P41NOX, P41NOXA, P41NOXB, P41NOXC, MGC20258, NADPH oxidase organizer 1, regulatory protein P41NOX, SH3PXD5, SNX28, Nox organizer 1

Gene ID: 124056

NCBI Accession: NP_653204, NP_751907