VPS28 antibody (C-Term)

Catalog No. ABIN185184

Product Details anti-VPS28 Antibody

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: VPS28

Sequence: ESAYNAFNRF LHA

Specificity: This antibody is expected to recognize isoform 1 (NP_057292.1) only.

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ESAYNAFNRFLHA, from the C Terminus of the protein sequence according to NP_057292.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration: 10 μg/mL.

Western Blot: Approx 24 kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25 kDa according to NP_057292.1). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VPS28

Target: VPS28 (Vacuolar Protein Sorting 28 (VPS28))

Alternative Name: VPS28 (VPS28 Products)

Background: VPS28, vacuolar protein sorting 28 (yeast), vacuolar protein sorting 28 homolog (S. cerevisiae), MGC60323, vacuolar protein sorting 28, yeast class E protein Vps28p homolog

Gene ID: 51160, 66914

NCBI Accession: NP_057292