SH3BP1 antibody (C-Term)

Catalog No. ABIN185183

Product Details anti-SH3BP1 Antibody

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SH3BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: SH3BP1

Sequence: PQPRPRSLAS ETN

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SH3BP1 antibody (ABIN185183)

Parrini, Sadou-Dubourgnoux, Aoki, Kunida, Biondini, Hatzoglou, Poullet, Formstecher, Yeaman, Matsuda, Rossé, Camonis: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).

Target Details for SH3BP1

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Alternative Name: SH3BP1 (SH3BP1 Products)

Synonyms: ARHGAP43 antibody, 3BP-1 antibody, SH3BP1 antibody, SH3 domain binding protein 1 antibody, SH3-domain binding protein 1 antibody, SH3 domain-binding protein 1 antibody, SH3BP1 antibody, Sh3bp1 antibody, LOC100398528 antibody

Background: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981

Gene ID: 23616

NCBI Accession: NP_061830