VPS35 antibody (C-Term)

Catalog No. ABIN185182

Product Details anti-VPS35 Antibody

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Purpose: VPS35 / MEM3

Sequence: SPESEGPIYE GLIL

Specificity: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-VPS35 antibody (ABIN185182)

Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." in: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35 (VPS35 Products)

Background: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35

Gene ID: 55737, 65114

NCBI Accession: NP_060676