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STK35 antibody (C-Term)

Verified STK35 Reactivity: Human, Mouse, Pig WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185178
  • Target See all STK35 Antibodies
    STK35 (Serine/threonine Kinase 35 (STK35))
    Binding Specificity
    • 15
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 26
    • 17
    • 11
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Pig
    Host
    • 41
    Goat
    Clonality
    • 41
    Polyclonal
    Conjugate
    • 12
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This STK35 antibody is un-conjugated
    Application
    • 30
    • 15
    • 13
    • 13
    • 9
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    Clik1 / STK35
    Sequence
    ELETRMDQVT CAA
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence ELETRMDQVTCAA, from the C Terminus of the protein sequence according to NP_543026.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasmic staining of Leydig cells. Recommended concentration: 10 μg/mL.

    Western Blot: Approx 55 kDa band observed in Human Breast cancer and in Mouse and Pig Testis lysates, and approx. 58 kDa in nuclear lysates of cell line HEK293 (calculated MW of 58.1 kDa according to Human NP_543026.2, 59.5 kDa according to Mouse NP_899085.3

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Goyal, Behring, Kumar, Siess: "Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates." in: PLoS ONE, Vol. 4, Issue 9, pp. e6981, (2009) (PubMed).

  • Target
    STK35 (Serine/threonine Kinase 35 (STK35))
    Alternative Name
    STK35 (STK35 Products)
    Synonyms
    clik1 antibody, stk35l1 antibody, MGC69346 antibody, im:6905214 antibody, zgc:153287 antibody, MGC115406 antibody, CLIK1 antibody, STK35L1 antibody, 1700054C12Rik antibody, Stk35l1 antibody, serine/threonine kinase 35 antibody, serine/threonine kinase 35 S homeolog antibody, stk35 antibody, stk35.S antibody, STK35 antibody, Stk35 antibody
    Background
    STK35, Clik1, bA550O8.2, bA550O8.2 CLIK1, serine/threonine kinase 35, CLP-36 interacting kinase, CLIK1, STK35L1, OTTHUMP00000030006, serine threonine kinase 35 long form
    Gene ID
    140901, 67333
    NCBI Accession
    NP_543026
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