RANGAP1 antibody (N-Term)

Catalog No. ABIN185170

Product Details anti-RANGAP1 Antibody

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RANGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: RANGAP1

Sequence: ASEDIAKLAE TLAK

Specificity: Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence ASEDIAKLAETLAK-C, from the N Terminus of the protein sequence according to NP_002874.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration: 2-4 μg/mL.

Western Blot: Approx 70 kDa band observed in 3T3 lysates (predicted MW of 68 kDa according to NP_002874). Recommended for use at 0.1-0.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RANGAP1

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Alternative Name: RANGAP1 (RANGAP1 Products)

Background: RANGAP1, KIAA1835, Ran GTPase activating protein 1, Fug1, MGC20266, SD, OTTHUMP00000198756, OTTHUMP00000198757, segregation distorter homolog, segregation distortion

Gene ID: 5905, 19387

NCBI Accession: NP_002874

Pathways: M Phase, Protein targeting to Nucleus