KALRN antibody (C-Term)

Catalog No. ABIN185110

Product Details anti-KALRN Antibody

Target: KALRN (Kalirin, RhoGEF Kinase (KALRN))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KALRN antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Kalirin

Sequence: RWHLGPGDPF STYV

Specificity: This antibody is expected to recognise NP_003938.1,(isoform 2),NP_001309918..1 (9isoform 5), NP_001309919.1,(isoform 6), NP_001309920.1 (isoform 7), and NP_001309921.1 (isoform 8).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4-5 μg/mL.

Western Blot: Preliminary testing showed a band at approx.. 200 kDa in Rat Brain lysate, after 0.1 μg/mL antibody staining (calculated Mwt. of 192.5 kDa according to XP_038944588.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KALRN

Target: KALRN (Kalirin, RhoGEF Kinase (KALRN))

Alternative Name: KALRN (KALRN Products)

Background: KALRN, kalirin, RhoGEF kinase, FLJ16443, HAPIP, TRAD, duo, huntingtin-associated protein interacting protein (duo), serine/threonine kinase with Dbl- and pleckstrin homology domains, hCG_2022551, DUET, DUO, FLJ12332, FLJ18196, FLJ18623,

Molecular Weight: 192kDa

Gene ID: 8997, 545156, 84009

NCBI Accession: NP_003938

Pathways: Neurotrophin Signaling Pathway