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KALRN antibody (C-Term)

Verified KALRN Reactivity: Human ELISA, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185110
  • Target See all KALRN Antibodies
    KALRN (Kalirin, RhoGEF Kinase (KALRN))
    Binding Specificity
    • 15
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 27
    • 6
    • 6
    • 1
    Human
    Host
    • 27
    Goat
    Clonality
    • 27
    Polyclonal
    Conjugate
    • 10
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KALRN antibody is un-conjugated
    Application
    • 13
    • 13
    • 11
    • 8
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    Kalirin
    Sequence
    RWHLGPGDPF STYV
    Specificity
    This antibody is expected to recognise NP_003938.1,(isoform 2),NP_001309918..1 (9isoform 5), NP_001309919.1,(isoform 6), NP_001309920.1 (isoform 7), and NP_001309921.1 (isoform 8).
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4-5 μg/mL.

    Western Blot: Preliminary testing showed a band at approx.. 200 kDa in Rat Brain lysate, after 0.1 μg/mL antibody staining (calculated Mwt. of 192.5 kDa according to XP_038944588.1). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    KALRN (Kalirin, RhoGEF Kinase (KALRN))
    Alternative Name
    KALRN (KALRN Products)
    Background
    KALRN, kalirin, RhoGEF kinase, FLJ16443, HAPIP, TRAD, duo, huntingtin-associated protein interacting protein (duo), serine/threonine kinase with Dbl- and pleckstrin homology domains, hCG_2022551, DUET, DUO, FLJ12332, FLJ18196, FLJ18623,
    Molecular Weight
    192kDa
    Gene ID
    8997, 545156, 84009
    NCBI Accession
    NP_003938
    Pathways
    Neurotrophin Signaling Pathway
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