GRAF antibody (C-Term)

Catalog No. ABIN185109

Product Details anti-GRAF Antibody

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: GRAF / ARHGAP26

Sequence: KTGLIPENYV EFL

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1, NP_001129080.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 92.2 kDa according to NP_055886.1). Recommended concentration: 0.3-1 μg/mL. An additional strong band of unknown identity was also consistently observed at 16 kDa. This band

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRAF

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Alternative Name: ARHGAP26 (ARHGAP26 Products)

Background: ARHGAP26, GRAF, Rho GTPase activating protein 26 , KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp1

Gene ID: 23092, 71302

NCBI Accession: NP_055886, NP_001129080