GRAF antibody (C-Term)

Catalog No. ABIN185109

Product Details anti-GRAF Antibody

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: GRAF / ARHGAP26

Sequence: KTGLIPENYV EFL

Specificity: This antibody is expected to recognise both reported isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KTGLIPENYVEFL, from the C Terminus of the protein sequence according to NP_055886.1, NP_001129080.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 92.2 kDa according to NP_055886.1). Recommended concentration: 0.3-1 μg/mL. An additional strong band of unknown identity was also consistently observed at 16 kDa. This band

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRAF

Target: GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))

Alternative Name: ARHGAP26 (ARHGAP26 Products)

Synonyms: ARHGAP26 antibody, GRAF antibody, GRAF1 antibody, OPHN1L antibody, OPHN1L1 antibody, 1810044B20Rik antibody, 2610010G17Rik antibody, 4933432P15Rik antibody, AI853435 antibody, mKIAA0621 antibody, Rho GTPase activating protein 26 antibody, ARHGAP26 antibody, Arhgap26 antibody

Background: ARHGAP26, GRAF, Rho GTPase activating protein 26 , KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp1

Gene ID: 23092, 71302

NCBI Accession: NP_055886, NP_001129080