PTBP1 antibody (N-Term)

Catalog No. ABIN185079

Product Details anti-PTBP1 Antibody

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PTBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: PTBP1 / PTB

Sequence: DGIVPDIAVG TKR

Specificity: This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence DGIVPDIAVGTKR-C, from the N Terminus of the protein sequence according to NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 55 kDa band observed in Mouse Ovary lysates and in lysates of cell line NIH3T3, K562, KNRK and Nuclear Jurkat (calculated MW of 57.2 kDa according to Human NP_114368.1 and 56.9 kDa according to Mouse NP_032982.2 and Rat NP_071961.2).

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PTBP1

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Alternative Name: PTBP1 (PTBP1 Products)

Background: PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I),

Gene ID: 19205, 29497

NCBI Accession: NP_002810, NP_114367, NP_114368, NP_787041

Pathways: Regulation of Muscle Cell Differentiation