FBXL2 antibody (N-Term)

Catalog No. ABIN185053

Product Details anti-FBXL2 Antibody

Target: FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXL2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: FBL2 / FBXL2

Sequence: VFSNNDEGLI NKK

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence VFSNNDEGLINKK-C, from the N Terminus of the protein sequence according to NP_036289.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Cortex. Recommended concentration: 8 μg/mL.

Western Blot: Preliminary testing showed a band at approx 48 kDa in Human Kidney lysate after 0.1 μg/mL antibody staining (calculated MW of 47.1 kDa according to NP_036289.2). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FBXL2

Target: FBXL2 (F-Box and Leucine-Rich Repeat Protein 2 (FBXL2))

Alternative Name: FBXL2 (FBXL2 Products)

Synonyms: FBL2 antibody, FBL3 antibody, 2810423A21Rik antibody, Fbl3 antibody, RGD1562243 antibody, fbxl20 antibody, sb:cb783 antibody, zgc:73374 antibody, Fbl2 antibody, Fbxl2 antibody, F-box and leucine rich repeat protein 2 antibody, F-box and leucine-rich repeat protein 2 antibody, F-box and leucine-rich repeat protein 20 antibody, FBXL2 antibody, Fbxl2 antibody, fbxl2 antibody, Fbxl20 antibody

Background: FBXL2, FBL2, F-box and leucine-rich repeat protein 2, FBL3, DKFZP564P0622, F-box protein containing leucine-rich repeats

Gene ID: 25827

NCBI Accession: NP_036289