NUP50 antibody (C-Term)
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- Target See all NUP50 Antibodies
- NUP50 (Nucleoporin 50kDa (NUP50))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NUP50 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- Npap60 / Nup50
- Sequence
- ELHKILLEKK DA
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).
- Cross-Reactivity
- Cow, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.
- Isotype
- IgG
- Top Product
- Discover our top product NUP50 Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.
Western Blot: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).
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Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).
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- Target
- NUP50 (Nucleoporin 50kDa (NUP50))
- Alternative Name
- NUP50 (NUP50 Products)
- Background
- NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530
- Gene ID
- 10762, 18141
- NCBI Accession
- NP_009103, NP_705931
- Pathways
- Tube Formation
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