TRIM21 antibody (C-Term)
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- Target See all TRIM21 Antibodies
- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This TRIM21 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- SSA1 / Ro52
- Sequence
- CPLNIGSQGS TDY
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.
- Isotype
- IgG
- Top Product
- Discover our top product TRIM21 Primary Antibody
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anti-Tripartite Motif Containing 21 (TRIM21) (AA 80-330) antibody
TRIM21 Reactivity: Human WB, IHC, IP, IF Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) antibodyTRIM21 Reactivity: Human WB, IP, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 268-465) antibodyTRIM21 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 301-400) antibodyTRIM21 Reactivity: Human, Mouse WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (C-Term) antibodyTRIM21 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 1-475) antibodyTRIM21 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 1-475) antibodyTRIM21 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 2C9 unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 1-475) antibodyTRIM21 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 68-175) antibodyTRIM21 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated
anti-Tripartite Motif Containing 21 (TRIM21) (AA 1-475) antibodyTRIM21 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antib
Peptide ELISA: antibody detection limit dilution 1:16000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- TRIM21 (Tripartite Motif Containing 21 (TRIM21))
- Alternative Name
- TRIM21 (TRIM21 Products)
- Background
- TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52 kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome ant
- Gene ID
- 6737
- NCBI Accession
- NP_003132
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