RNF13 antibody (C-Term)
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- Target See all RNF13 Antibodies
- RNF13 (Ring Finger Protein 13 (RNF13))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This RNF13 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Purpose
- RNF13 / RZF (isoform 1 and 3)
- Sequence
- PNGERDYNIA NTV
- Specificity
- Reported variants represent identical protein (NP_009213.1, NP_899237.1).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-PNGERDYNIANTV, from the C Terminus of the protein sequence according to NP_009213.1.
- Isotype
- IgG
- Top Product
- Discover our top product RNF13 Primary Antibody
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- Application Notes
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Immunohistochemistry: This antibody stained islets but not in ducts and acini of Human normal pancreas. However, in pancreatic ductal adenocarcinoma staining was in the ducts and acini.
Western Blot: Approx. 42 kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45 kDa in lysates of cell line HeLa and in preliminary testing of U251 cell lysate (calculated MW of 42.8 kDa according to
Peptide ELISA: antibody detection limit dilution 1:1000.
- Comment
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Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- RNF13 (Ring Finger Protein 13 (RNF13))
- Alternative Name
- RNF13 (RNF13 Products)
- Synonyms
- RZF antibody, 2010001H16Rik antibody, Rzf antibody, ring finger protein 13 antibody, ring finger protein 13 L homeolog antibody, RNF13 antibody, Rnf13 antibody, rnf13.L antibody
- Background
- RNF13, ring finger protein 13, RZF, RING zinc finger protein, MGC13689, FLJ93817
- Gene ID
- 11342
- NCBI Accession
- NP_009213
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