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ARIH2 antibody (C-Term)

Verified ARIH2 Reactivity: Human, Mouse WB, ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN184916
  • Target See all ARIH2 Antibodies
    ARIH2 (protein ariadne-2 homolog (ARIH2))
    Binding Specificity
    • 9
    • 8
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 48
    • 20
    • 8
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Mouse
    Host
    • 44
    • 4
    Goat
    Clonality
    • 45
    • 2
    Polyclonal
    Conjugate
    • 27
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARIH2 antibody is un-conjugated
    Application
    • 35
    • 26
    • 23
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Purpose
    ARIH2 / TRIAD1
    Sequence
    EQRRRTLLKD FHDT
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EQRRRTLLKDFHDT, from the C Terminus of the protein sequence according to NP_006312.1.
    Isotype
    IgG
    Top Product
    Discover our top product ARIH2 Primary Antibody
  • Application Notes

    Western Blot: Approx 60 kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8 kDa according to NP_006312.1) Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60 kDa in lys

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Comment

    Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Raz, Buijze, Raz, Verwey, Anvar, Aartsma-Rus, van der Maarel: "A novel feed-forward loop between ARIH2 E3-ligase and PABPN1 regulates aging-associated muscle degeneration." in: The American journal of pathology, Vol. 184, Issue 4, pp. 1119-31, (2014) (PubMed).

  • Target
    ARIH2 (protein ariadne-2 homolog (ARIH2))
    Alternative Name
    ARIH2 (ARIH2 Products)
    Synonyms
    ARI2 antibody, TRIAD1 antibody, AI843547 antibody, UIP48 antibody, wu:fa09c12 antibody, zgc:64093 antibody, ariadne RBR E3 ubiquitin protein ligase 2 antibody, ariadne RBR E3 ubiquitin protein ligase 2 L homeolog antibody, ariadne homolog 2 (Drosophila) antibody, ARIH2 antibody, Arih2 antibody, arih2.L antibody, arih2 antibody
    Background
    ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
    Gene ID
    10425, 23807
    NCBI Accession
    NP_006312
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