NLRP3 antibody (C-Term)

Catalog No. ABIN184887

Product Details anti-NLRP3 Antibody

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: CIAS1 / cryopyrin ( C Terminus)

Sequence: EKPELTVVFE PSW

Specificity: Variants (NP_001073289.1, NP_004886.3) encode the same isoform.

Cross-Reactivity: Cow, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EKPELTVVFEPSW, from the C Terminus of the protein sequence according to NP_001073289.1, NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 4 μg/mL.

Western Blot: This antibody is not recommended for use in Western blotting.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and plasma membrane of U937 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentra

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3 (NLRP3 Products)

Background: NLRP3, CIAS1, FCU, MWS, FCAS, NALP3, C1orf7, PYPAF1, AII/AVP, AGTAVPRL, CRYOPYRIN, cold autoinflammatory syndrome 1, cryopyrin, Muckle-Wells syndrome, chromosome 1 open reading frame 7, PYRIN-containing APAF1-like protein 1, neuronal apoptosis inhibitor p

Gene ID: 114548

NCBI Accession: NP_001073289, NP_004886, NP_899632, NP_001120933, NP_001120934

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome