Lysine (K)-Specific Methyltransferase 2B (KMT2B) (N-Term) antibody

Catalog No. ABIN184880

Product Details

Target: Lysine (K)-Specific Methyltransferase 2B (KMT2B)

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: KMT2D/MLL2

Sequence: DSQNLAGEDK DSQ

Specificity: The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3.

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence DSQNLAGEDKDSQ-C, from the N Terminus of the protein sequence according to NP_003473.3.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Using our routine western blotting protocol we would not expect to reliably detect proteins larger than 250 kDa (calculated MW of approx. 593KDa according to NP_003473.3).

DS IHC Results: In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts . Similar nuclear patches are observed in Human Liver. Recommended concentration: 4-8 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: Lysine (K)-Specific Methyltransferase 2B (KMT2B)

Alternative Name: KMT2D (KMT2B Products)

Synonyms: HRX2 antibody, MLL1B antibody, MLL2 antibody, MLL4 antibody, TRX2 antibody, WBP7 antibody, lysine methyltransferase 2B antibody, KMT2B antibody

Background: MLL2, myeloid/lymphoid or mixed-lineage leukemia 2, ALR, ALL1-related gene, AAD10, CAGL114, KMT2D, MLL4, TNRC21, ALL1-related, trinucleotide repeat containing 21, KMS, KABUK1, Lysine methyltransferase 2D

Gene ID: 8085

NCBI Accession: NP_003473

Pathways: Warburg Effect