HSPB8 antibody (C-Term)

Catalog No. ABIN184852

Product Details anti-HSPB8 Antibody

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HSPB8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: H11 / HSP22

Sequence: NELPQDSQEV TCT

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence NELPQDSQEVTCT, from the C Terminus of the protein sequence according to NP_055180.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Adrenal Gland and Tonsil. Recommended concentration: 10 μg/mL.

Western Blot: Approx 20-25 kDa band seen in Human Skeletal Muscle lysate [Predicted MW of approx. 22 kDa according to NP_055180]. Recommended for use at 0.5-1 μg/mL. Primary incubated for 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Alternative Name: HSPB8 (HSPB8 Products)

Background: HSPB8, heat shock 22 kDa protein 8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22, Charcot-Marie-Tooth disease, spinal, heat shock 27 kDa protein 8, hereditary motor neuropathy, distal, protein kinase H11, small stress protein-like protein HSP22, spinal musc

Gene ID: 26353

NCBI Accession: NP_055180