ARL6IP5 antibody (C-Term)

Catalog No. ABIN184829

Product Details anti-ARL6IP5 Antibody

Target: ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARL6IP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: GTRAP3-18 / JWA

Sequence: NRLTDYISKV KE

Specificity: C2 passed G1 as 20 kDa in HBR, C2P2 fail in _enh, try HBAM, HBCR, HBFC BP08/10/2021, 22+48 kDa in HBAM-B104, faint 48 kDa in HBAM-B201, multiple bannd in > 37 kDa in both HBCR, multiple bands inclu 22 kDa in both HBFC, opt in HBAM-B201 at 2 μg/mL , rpt HBAM-B10

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NRLTDYISKVKE, from the C Terminus of the protein sequence according to NP_006398.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 22 kDa band observed in Human Cerebellumn lysates and in lysates of cell line K562 (calculated MW of 21.6 kDa according to NP_006398.1). An additional band of 75-100 kDa was also observed in some lysates.and was successfully blocked by

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER/ plasma membranes and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ARL6IP5 antibody (ABIN184829)

Wang, Gong, Chen, Liu, Li, Li, Zhou: "JWA regulates XRCC1 and functions as a novel base excision repair protein in oxidative-stress-induced DNA single-strand breaks." in: Nucleic acids research, Vol. 37, Issue 6, pp. 1936-50, (2009) (PubMed).

Target Details for ARL6IP5

Target: ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))

Alternative Name: ARL6IP5 (Arl6ip5 Products)

Synonyms: zp22 antibody, addicsin antibody, zgc:92811 antibody, wu:fj55a04 antibody, arl6ip5 antibody, cb937 antibody, zgc:101611 antibody, jmx antibody, jwa antibody, hp22 antibody, praf3 antibody, derp11 antibody, hspc127 antibody, gtrap3-18 antibody, 5930404D22Rik antibody, AV001879 antibody, Aip-5 antibody, Gtrap3-18 antibody, addiscin antibody, DERP11 antibody, GTRAP3-18 antibody, JWA antibody, PRAF3 antibody, ADP-ribosylation factor-like 6 interacting protein 5b antibody, ADP-ribosylation factor-like 6 interacting protein 5a antibody, ADP ribosylation factor like GTPase 6 interacting protein 5 antibody, ADP-ribosylation factor-like 6 interacting protein 5 antibody, ADP-ribosylation factor like GTPase 6 interacting protein 5 antibody, arl6ip5b antibody, arl6ip5a antibody, arl6ip5 antibody, Arl6ip5 antibody, ARL6IP5 antibody

Background: ARL6IP5, GTRAP3-18, JWA, ADP-ribosylation-like factor 6 interacting protein 5, DERP11, HSPC127, PRAF3, addicsin, hp22, jmx, PRA1 domain family 3, cytoskeleton related vitamin A responsive protein, dermal papilla derived protein 11, glutamate transporter E

Gene ID: 10550

NCBI Accession: NP_006398

Pathways: Dicarboxylic Acid Transport