NDC80 antibody (C-Term)

Catalog No. ABIN184821

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: HEC1

Sequence: YEKKATLIKS SEE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-YEKKATLIKSSEE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75-80 kDa band observed in Hela lysate (predicted MW of 74 kDa according to NP_006092). Recommended for use at 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 (NDC80 Products)

Background: NDC80, HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1, KNTC2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3, hsNDC80, kinetochore associated 2

Gene ID: 10403

NCBI Accession: NP_006092

Pathways: Maintenance of Protein Location